NeuroMorpho.Org is the largest centrally curated online repository of digital reconstructions of axonal and dendritic morphologies.

This public resource freely provides light and electron microscopy tracings contributed by more than 220 labs worldwide from over 500 publications.

Prominent research efforts are unveiling how circuits constitute the basic functional units of nervous systems.

Among them, Drosophila is currently the species with more promising results in mapping brain-wide connections at the
individual neuron level. The pioneering FlyCircuit Database has already traced and co-registered the neurite wiring corresponding to approximately 10% (v1.0) and 23% (v1.1) of the cells in the Drosophila brain.

We present the definition of a novel multichannel file structure and corresponding Vaa3D plug-in to handle this new type of data. We also introduce a design to tag dynamic structural changes in a time-coded manner. Next, we illustrate ongoing progress in using the multichannel/time-lapse system on developing neurons in the Drosophila larva. Time-varying images of overall neuronal morphology along with fluorescently labeled subcellular cytoskeletal components are digitally traced into the aforementioned file structures. These new reconstructions enable complete statistical analysis of the structural changes and the underlying molecular processes.